At Amplifye, we believe optimal nutrition begins with making food work better for the body. That’s why we partnered with Biorbis, a leading third-party biotech lab, to rigorously evaluate the effect of our protein enzyme, P24, on protein digestion and amino acid bioaccessibility.

Study Objectives 

Here’s what we set out to do: 

• Measure the increase in protein bioaccessibility and how effectively the body can break down and use proteins, across 19 sources (including animal, dairy, and plant-based proteins).
  

• Determine which amino acids increased with the application of P24 and understand the associated benefits for human health.

Methodology 

To do this, researchers applied validated methods including the TNBS assay to measure hydrolysis degree (DH%) and HPLC with fluorescence detection to quantify amino acid concentration in milligrams per gram of protein. This was accomplished with the INFOGEST Model 2.0, an industry-standard methodology that simulates stomach digestion. 

Key Terminology 

• Bioaccessibility: The extent to which proteins can be absorbed in the digestive tract.
  

• Amino Acids: The building blocks of protein. Essential amino acids are only found in food and are vital for human health.
  

• Amino Acid Profile: The spectrum and concentration of amino acids within a protein source.
  

• Hydrolysis Degree (DH%): A measure of protein breakdown, indicating how many small peptides and amino acids were released during digestion.

How does bioaccessibility support human health?

Most people are surprised to learn that our bodies don't absorb all the protein we eat, leaving important nutrients behind. This is where bioaccessibility matters — improving it can significantly enhance how efficiently we use the nutrients we consume.

By increasing protein bioaccessibility and amino acid concentration starting in the stomach, P24 may support:

Digestive Comfort

As P24 kick-starts the digestive process, it’s easier for your body to break down proteins. This means less digestive strain, a healthy microbial balance, and enhanced gut lining integrity. 

Muscle Recovery

Any athlete knows protein is essential for muscle growth and recovery, but how? Protein stimulates muscle protein synthesis, reduces muscle breakdown, and replenishes amino acid stores. 

Deeper Sleep

Through delivering the amino acid tryptophan — a precursor to melatonin — protein supports deeper, more restful sleep. It also stabilizes blood sugar, aids muscle repair, and regulates stress hormones like cortisol.

Sustained Energy

If you’re looking for all-day energy (without that afternoon slump), protein may be your answer. Protein stabilizes blood sugar while increasing satiety and enhancing mitochondrial function — keeping you going from the cellular level.

Learn more about how P24 interacts with the digestive system to support your unique health goals here.

Key Study Findings

The study examined 20 protein sources, assessing the degree of protein breakdown (DH%) and the concentration of free amino acids after simulated gastric digestion with and without P24.

Each protein source (170g serving) was paired with one 200 mg capsule of P24.

Animal-Based Proteins

 

Chicken: 1.8x More Bioaccessible Protein with P24

Chicken is a versatile and affordable complete protein source that’s rich in all essential amino acids. The study shows chicken’s 1.8x bioaccessibility increase when paired with P24. Amino acid increases include:

+24.7% lysine: Linked to metabolic function, neurotransmitter and protein synthesis 

+25.7% leucine: Linked to muscle protein synthesis and immune function

Pork: 4.8x More Bioaccessible Protein with P24

Pork is a rich and flavorful protein, but is generally considered hard to digest and low in nutritional value. The study revealed a 4.8x increase in protein bioaccessibility when pork was paired with P24. Amino acid increases include: 

+166% lysine: Linked to metabolic function, neurotransmitter and protein synthesis 

+59.8% phenylalanine: Linked to neurotransmitter synthesis supporting cognition

+88.6% leucine: Linked to muscle protein synthesis and immune function 

 

Ground Beef: 2.5x More Bioaccessible Protein with P24 

Ground beef is a staple protein in many diets and known for its dense nutritional profile. With P24, ground beef showed a 2.5x improvement in bioaccessibility. Amino acid increases include: 

+44.8% lysine:  Linked to metabolic function, neurotransmitter and protein synthesis 

+35.6% phenylalanine: Linked to neurotransmitter synthesis supporting cognition 

 

Beef Jerky: 1.6x More Bioaccessible Protein with P24

Beef jerky, a high-protein, shelf-stable snack, showed a 1.6x increase in bioaccessibility when paired with P24. Amino acid increases include: 

+21.9% lysine: Linked to metabolic function, neurotransmitter and protein synthesis 

+16.4% phenylalanine: Linked to neurotransmitter synthesis supporting cognition 

+11.8% leucine: Linked to muscle protein synthesis and immune function 

 

Eggs: 1.8x More Bioaccessible Protein with P24 

Eggs are widely regarded as a complete protein, offering all nine essential amino acids. When consumed with P24, eggs demonstrated a 1.8x increase in bioaccessibility. Amino acid increases include: 

+35.6% leucine: Linked to muscle protein synthesis and immune function 

+21.6% lysine: Linked to metabolic function, neurotransmitter and protein synthesis 

+33.4% tyrosine: Linked to immune function, fat metabolism and collagen

 

Salmon: 2.0x More Bioaccessible Protein with P24 

Salmon is an omega-3-rich protein that also saw significant improvements with P24: a 2.0x increase in bioaccessibility. Amino acid increases include: 

+21.2% lysine: Linked to metabolic function, neurotransmitter and protein synthesis  

+47.1% leucine: Linked to muscle protein synthesis and immune function

+340.5% aspartic acid: Linked to endocrine and nervous system function

+40.2% tyrosine: Linked to immune function, fat metabolism and collagen 

 

Shrimp: 1.7x More Bioaccessible Protein with P24 

Shrimp, a low-fat seafood option, had a 1.7x boost in protein bioaccessibility with P24. Amino acid increases include: 

+54% lysine: Linked to metabolic function, neurotransmitter and protein synthesis  

+45.6% leucine: Linked to muscle protein synthesis and immune function 

+31.5% phenylalanine: Linked to neurotransmitter synthesis supporting cognition 

 

Tuna: 2.1x More Bioaccessible Protein with P24 

Tuna is known for its high protein content and lean profile. With P24, tuna exhibited a 2.1x increase in bioaccessibility. Amino acid increases include: 

+57.7% valine: Linked to muscle protein synthesis, cellular energy, and endurance 

+8.5% lysine: Linked to metabolic function, neurotransmitter and protein synthesis  

+8.2% histidine: Linked to immune and sexual function, digestive enzyme production

 

Sea Bass: 2.0x More Bioaccessible Protein with P24 

Sea bass, a delicate white fish, experienced a 2.0x increase in bioaccessibility when paired with P24. Amino acid increases include: 

+16.5% isoleucine: Linked to energy and blood sugar regulation, as well as muscle recovery

+36.6% threonine: Linked to immune function, metabolism, mucosal integrity, and collagen synthesis 

+21.3% tyrosine: Linked to immune function, fat metabolism and collagen 

 

 Dairy-Based Proteins

 

Cottage Cheese: 1.7x More Bioaccessible Protein with P24 

Cottage cheese is a dairy-based protein known for its slow-digesting casein content, making it ideal for overnight recovery. When combined with P24, it showed a 1.7x increase in bioaccessibility. Amino acid increases include: 

+82% lysine: Linked to metabolic function, neurotransmitter and protein synthesis  

+21.5% tyrosine: Linked to immune function, fat metabolism and collagen 

+49.5% isoleucine: Linked to energy and blood sugar regulation, as well as muscle recovery

 

Greek Yogurt: 1.5x More Bioaccessible Protein with P24 

Greek yogurt is popular for its high protein density and probiotic benefits. The study revealed a 1.5x increase in bioaccessibility with P24. Amino acid increases include:

+57.9% isoleucine: Linked to energy and blood sugar regulation, as well as muscle recovery

+15.7% leucine: Linked to muscle protein synthesis and immune function 

+18.3% tyrosine: Linked to immune function, fat metabolism and collagen

Milk: 1.7x More Bioaccessible Protein with P24 

Milk is a widely consumed complete protein offering an array of essential amino acids. In the study, pairing milk with P24 resulted in a 1.7x increase in protein bioaccessibility. Amino acid increases include: 

+15.4% leucine: Linked to muscle protein synthesis and immune function 

+8.8% phenylalanine: Linked to neurotransmitter synthesis supporting cognition

+20.2% tyrosine: Linked to immune function, fat metabolism and collagen 

 

Whey: 1.5x More Bioaccessible Protein with P24 

Whey is one of the fastest-absorbing proteins and a go-to for athletes. With P24, its bioaccessibility improved by 1.5x. Amino acid increases include:

+1,614% histidine: Linked to immune and sexual function, digestive enzyme production

+51.1% isoleucine: Linked to energy and blood sugar regulation, as well as muscle recovery 

+158.2% aspartic acid: Linked to endocrine and nervous system function 

Mozzarella: 1.9x More Bioaccessible Protein with P24 

Mozzarella is a softer dairy protein with notable nutritional density. P24 enhanced its bioaccessibility by 1.9x. Amino acid increases include:

+9.6% histidine: Linked to immune and sexual function, digestive enzyme production 

 

Plant-Based Proteins

Plant proteins are generally harder for our bodies to digest compared to animal proteins due to their complex structure and the presence of natural compounds that inhibit digestion. Many plant-based proteins are encased in fibrous cell walls that our digestive enzymes struggle to break down. This reduced digestibility means the body may not absorb all the amino acids as efficiently as it does from animal sources — and why P24 offers so much potential to the plant-based eater.

With P24, these complex plant proteins are more easily digested and our body can access a higher concentration of key amino acids. 

Tofu: 3.2x More Bioaccessible Protein with P24 

Tofu, a cornerstone of many plant-based diets, showed a 3.2x increase in bioaccessibility with P24—one of the highest in the study. Amino acid increases include: 

+16.3% phenlylalanine: Linked to neurotransmitter synthesis supporting cognition

 

Pea Powder: 2.7x More Bioaccessible Protein with P24 

Pea powder is a popular vegan protein supplement, and P24 elevated its bioaccessibility by 2.7x. Amino acid increases include: 

+36.1% lysine: Linked to metabolic function, neurotransmitter and protein synthesis

+31.5% leucine: Linked to muscle protein synthesis and immune function 

+83.1% tyrosine: Linked to immune function, fat metabolism, and collagen

Black Beans: 1.7x More Bioaccessible Protein with P24 

Black beans, rich in fiber and protein, demonstrated a 1.7x boost in bioaccessibility with P24. Amino acid increases include:

+19% phenylalanine: Linked to neurotransmitter synthesis supporting cognition

+38.2% lysine: Linked to metabolic function, neurotransmitter and protein synthesis 

+8.1% arginine: Linked to cardiovascular and immune function

Chickpeas: 2.1x More Bioaccessible Protein with P24 

Chickpeas are a versatile legume high in protein and fiber. When paired with P24, they showed a 2.1x bioaccessibility increase. Amino acid increases include:

+9.8% leucine: Linked to muscle protein synthesis and immune function

+8.1% phenylalanine: Linked to neurotransmitter synthesis supporting cognition

+4.8% arginine: Linked to cardiovascular and immune function

Brown Lentils: 2.1x More Bioaccessible Protein with P24 

Brown lentils, another plant-based staple, had a 2.1x bioaccessibility increase when combined with P24. Amino acid increases include:

+55.6% aspartic acid: Linked to endocrine and nervous system function 

+9.1% glutamine: Linked to immune function and gut health

 

Almonds: 1.9x More Bioaccessible Protein with P24 

Almonds, a plant protein rich in healthy fats, showed a 1.9x improvement in bioaccessibility. Amino acid increases include:

+71% arginine: Linked to cardiovascular and immune function 

+43.6% glutamine: Linked to immune function and gut health 

+10.8% glycine: Linked to gut health, nervous system function, and detoxification

 

Discussion 

This third party research illustrates just how powerfully the enzyme P24 unlocks the nutritional potential of everyday protein sources. Across both animal and plant proteins, P24:

• Reduces digestive stress by commencing protein digestion early in the stomach through precision hydrolysis.
  

• Increases bioaccessibility, releasing more nutrition per gram of food
  

• Amplifies amino acid concentration, delivering more of the essential compounds your body needs
  

• Enhances the functional profile of protein, allowing for targeted health benefits ranging from energy and mood to recovery and immune resilience

Whether you’re an athlete, a busy professional, or someone striving for better digestion and nutrition, P24 offers a unique, third-party tested solution to get more from the meals you’re already eating. By just taking a single capsule with your biggest meal of the day, you can unlock the full potential of protein. 

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